High-Taxol-Yield Taxus Cell Line

Relieves key inhibition

Import of miR5298b relieves the inhibition of taxol biosynthesis by the TcNPR3-TcTGA6 complex, providing a new route to high-yield Taxus material.

Chen, Ying · Zhang, Meng · Zhang, Wenli · Wang, Yamin · Zhang, Hua · Liu, Yang · Yu, Longjiang · Fu, Chunhua

International Journal of Biological Macromolecules 2023

Advantages

miR5298b promoted taxol accumulation in transient leaf overexpression experiments

miR5298b upregulated taxol biosynthesis genes including DBAT, TASY, and T5H

TcNPR3 repressed taxol biosynthesis

TcTGA6 directly binds the TGACG motif in promoters of TASY, T5H, and T10H to negatively regulate taxol biosynthesis