Doubled synthesis yield
Obtained in high yield via a one-pot multi-segment condensation strategy that eliminates tedious intermediate purification, providing a practical route to larger mirror-image proteins.
Shi, Chaowei · Cui, Tingting
Organic and Biomolecular Chemistry 2025
One-pot multi-segment ligation minimizes losses from intermediate isolation, achieving 9.8% overall yield, a twofold improvement over previous work.
One-pot three- and four-segment ligations eliminate the need to isolate and handle intermediates, streamlining the process.
Provides a practical and streamlined approach for synthesizing larger mirror-image proteins.