Mitochondrial A-to-G Base Editor

87% editing efficiency

Directed-evolved TadA-8e variants achieve efficient and precise A-to-G base editing in human mitochondrial DNA with greatly reduced off-target effects.

Chen, Liang · Hong, Mengjia · Changming, Luan · Yuan, Meng · Fang, Yue · Huang, Hao · Hongyi, Gao · Zhang, Dan · Chen, Xi · Yi, Zongyi · Liu, Mingyao · Wei, Wensheng · Gao, Liangcai · Song, Gaojie · Zhou, Xiaoming · Li, Dali

Nature Biotechnology 2025

Specifications

人细胞中编辑效率最高

{'zh': '87', 'en': ''} %

编辑平均提升

{'zh': '3.2', 'en': ''} 倍

大鼠胚胎注射靶向突变频率最高

{'zh': '44', 'en': ''} %

Advantages

Greatly reduced DNA and RNA off-target effects