Dual RNA labeling in live cells
A phenol derivative probe engineered for APEX2 proximity labeling that balances reactivity, hydrophilicity, and lipophilicity to enable efficient and specific RNA labeling in live cells, and can be combined orthogonally with singlet oxygen labeling.
Liang, Jiying · Gao, Xutao · Jia, Han · Li, Ran · Tse, Edmund C.M. · Li, Ying
Analytical Chemistry 2023
Enables high-sensitivity and high-specificity transcriptome analysis of the mitochondrial matrix
Validated orthogonality of APEX2/Ph_N₃ and DRAQ5-¹O₂ labeling at the imaging level