Low-Cholesterol BLAN Gene-Editing Carrier

Efficient DC editing

Low-cholesterol BLANs enable efficient CRISPR/Cas9 delivery to dendritic cells, overcoming a key gene-editing barrier.

Mao, Kuirong · Tan, Huizhu · Cong, Xiuxiu · Liu, Ji · Xin, Yanbao · Wang, Jialiang · Guan, Meng · Li, Jiaxuan · Zhu, Ge · Meng, Xiandi · Guojiao, Lin · Haorui, Wang · Jing, Han · Wang, Ming · Yang, Yongguang · Sun, Tianmeng

Acta Pharmaceutica Sinica B 2025

Advantages

Low-cholesterol-density BLANs exhibit exceptional mRNA uptake, improved endosomal escape, and efficient sgRNA release

BLANmCas9/gPD-L1 results in substantial PD-L1 knockout in conventional dendritic cells

BLANmCas9/gPD-L1 enhances cDC1 activation, T cell stimulation, and significantly suppresses tumor growth