LNA-Modified Split DNAzyme Probes

40-min fecal visual detection

Accurately captures C. difficile target dsDNA within RPA, enabling direct visual readout.

Chen, Zhuo · Yuexin, Yao · Ruiqin, Luo · Xia, Xianru · Danni, Wang · Li, Chengyu · Xiao-Ming, Sun

Sensors and Actuators, B: Chemical 2025

Specifications

Detection time

{'zh': '40', 'en': '40'} min

Advantages

Results within 40 min

RPA isothermal amplification combined with probe detection completes the entire process from sample to visual readout in 40 min.

Naked-eye readout

Split DNAzyme catalyzes oxidation of colorless ABTS to a green product, enabling direct visual observation or smartphone recording.

High specificity

LNA-modified probes bind target nucleic acid extremely stably, resisting dissociation by temperature or enzymes, and multiple split probes must assemble correctly to generate signal.

High sensitivity

The method has high sensitivity, though no specific values are given in the abstract.

Applications

• C. difficile diagnosis：For rapid screening of C. difficile in fecal samples to aid timely treatment.
• visual nucleic acid detection：Converts nucleic acid amplification into a color change visible to the naked eye, no instrument required.
• isothermal amplification assay：Amplifies at a constant temperature, eliminating thermal cycler dependence and suitable for point-of-care rapid testing.