Static droplet array chip

Quant in 3-5h, ID & AST in 12h

Directly from unprocessed blood, quantify pathogens and perform AST

Zhidian, Diao · Ge, Anle · Hao, Zhou · Xuan, Zhou · Kan, Lingyan · Wei, Gao · Wei, Shen · Ji, Yuetong · Wang, Hongwei · Xu, Jian · Wang, Xixian · Ma, Bo

Sensors and Actuators, B: Chemical 2025

Specifications

Detection limit

{'zh': '10', 'en': '10'} CFU/mL

Bacterial quantification time

{'zh': '3–5', 'en': '3–5'} h

Total detection time

{'zh': '12', 'en': '12'} h

Advantages

No pretreatment

The chip captures and quantifies pathogens directly from unprocessed blood, eliminating culture and purification steps.

Quantification in 3-5 h

The microchamber array isolates individual bacteria, enabling quantification within 3–5 h after cell lysis and medium exchange.

AI-accelerated AST

Artificial intelligence image recognition is employed for rapid assessment of AST, significantly reducing the time required compared to conventional methods.

Applications

• Bloodstream infection diagnosis：Direct quantification of pathogens from unprocessed blood shortens diagnosis time.
• Point-of-care testing：The chip-based assay is simple and rapid, suitable for POC settings.
• Antimicrobial susceptibility testing：AI image recognition accelerates AST readout for targeted therapy.
• Rapid pathogen identification：Cultured pathogens can be exported for subsequent strain identification.