Dual-Protease-Triggered Chemiluminescent Probe

382-fold signal boost for liver cancer imaging

This probe is sequentially activated by two proteases linked to liver cancer, enabling specific imaging of liver cancer cells and avoiding false positives from single-enzyme detection.

Zhihui, Zhang · Sun, Ru · Bian, Chong · Wang, Hongbo · Zhao, Zhen · Lv, Panpan · Lu, Jianzhong · Haixin, Zhang · Zeng, Hulie · Yuanyuan, Chen · Cao, Zhijuan

Chinese Chemical Letters 2025

Advantages

382-fold enhancement of chemiluminescent emission upon dual-protease activation

Rapid response within several minutes

High sensitivity

Good cell viability