Near-Infrared Fluorescent Probe

Dual-Channel Discrimination of Bio-thiols

An acridine-conjugated indole salt-based probe that distinguishes Cys, Hcy, and GSH via dual-channel and time-dependent fluorescence, addressing the challenge of discriminating structurally similar bio-thiols.

Min, Zhou · Lin, Yanfei · Bai, Tianwen · Tianqing, Ye · Zeng, Yanbo · Li, Lei · Zhaosheng, Qian · Guo, Longhua · Liu, Haiying · Wang, Jianbo

Sensors and Actuators, B: Chemical 2024

Specifications

Hcy
{'zh': '654', 'en': ''} nm

Advantages

Under 488 nm excitation, significant turn-on NIR fluorescence response toward Cys at 654 nm with a large Stokes shift of about 166 nm

Under 640 nm excitation, turn-on NIR fluorescence response for Hcy/GSH at 695 nm

Successful intracellular imaging of endogenous and exogenous bio-thiols in living cells