A genome-wide sgRNA library based on an adenine base editor for systematic perturbation of serine, threonine, and tyrosine residues at endogenous loci, enabling functional profiling of amino acids.
Li, Yizhou · Xu, Tao · Ma, Huazheng · Yue, Di · Liu, Ying · Zhou, Zhuo · Wei, Wensheng
Nature Chemical Biology 2024
Contains 817,089 sgRNAs targeting 584,337 S/T/Y sites
Identified 3,467 functional substitutions affecting cell fitness
677 substitutions involve phosphorylation
Found 309 enriched sites potentially as cancer driver mutations