Light-controlled spatiotemporal editing
This circular gRNA uses pre-installed photocleavable groups and intramolecular cyclization to enable light-gated gene editing, avoiding the extensive screening required for heavily modified linear gRNAs.
Sun, Yingjie · Wen-Da, Chen · Liu, Ji · Li, Junjin · Zhang, Yu · Cai, Weiqi · Liu, Li · Tang, Xinjing · Hou, Jian · Wang, Ming · Cheng, Liang
Angewandte Chemie - International Edition 2023
without notable editing leakage
precise cleavage of GFP and VEGFA genes in live cells
light-mediated MSTN gene editing in embryos with excellent OFF/ON switch efficiency