Core-shell magnetic beads

23-fold signal enhancement

The surface polymer brush thickness and grafting density are tunable, significantly improving antibody loading and interfacial sensing performance.

Nie, Yingrui · Zhimin, Zhao · Chen, Xinyu · Wang, Fan · Huang, Juan · Jiang, Yong

Langmuir 2026

Specifications

Chemiluminescence enhancement

{'zh': '23', 'en': '23'} ×

Signal-to-background ratio

{'zh': '575', 'en': '575'}

Limit of detection

{'zh': '0.11', 'en': '0.11'} ng/mL

Number of clinical plasma samples

{'zh': '50', 'en': '50'}

Grafting density range

{'zh': '0.05–0.48', 'en': '0.05–0.48'} chains/nm²

Advantages

23-fold signal boost

Optimized beads achieve a 23-fold enhancement in chemiluminescence intensity through improved antibody loading and interfacial performance.

Higher signal-to-background

For CK-MB detection, the signal-to-background ratio increases from 79 to 575, greatly reducing background interference.

LOD of 0.11 ng/mL

The method provides a limit of detection of 0.11 ng/mL for CK-MB, meeting clinical detection needs.

Validated in clinical samples

The method was validated using 50 clinical plasma samples, demonstrating robustness for practical diagnostic applications.

Applications

• In vitro diagnostics：Enables highly sensitive detection of cardiac marker CK-MB for acute myocardial infarction diagnosis.
• Immunoassay：Provides a chemiluminescence immunoassay platform with high signal-to-background ratio for improved accuracy.
• Antibody immobilization：Enables efficient antibody conjugation via high-density carboxyl groups while preserving activity.
• Functionalized colloids：Serves as a functionalized colloidal carrier for signal amplification in diagnostic reagents.