Cuts at sulfated domains
Unlike terrestrial heparinases, HD1492 preferentially cleaves sulfated regions, leaving resistant oligosaccharides with nonsulfated disaccharides linked to an N-sulfated disaccharide, thereby revealing nonsulfated domain distribution in HP/HS.
Xu, Xiangyu · Zhang, Qingdong · Xiaoyu, Cui · Liu, Yuan · Xu, Yingying · Wang, Xu · Wei, Lin · Ruiqing, Cheng · Wang, Wenshuang · Zhang, Xiying · Li, Fuchuan
Journal of Agricultural and Food Chemistry 2025
Preferentially cleaves sulfated regions in HP/HS
Generates resistant oligosaccharides from low-sulfated HS
Reveals distribution of nonsulfated domains in mouse organ-derived GAGs and the drug sulodexide