Distinguishes Arg dimethylation isomers
By tuning pH to control reactivity with methylglyoxal, it simultaneously enriches and distinguishes asymmetric and symmetric arginine dimethylation isomers in complex cell samples without prior separation.
Wang, Jiayi · Liu, Ye · Wang, Qi · Ma, Yanni · Longrun, Shi · Wang, Yan · Li, Zhouxian · Fang, Zheng · Liu, Zhen · Yan, Jiayang · Mu, Xia · Li, Guohui · Wang, Anhui · Wang, Keyun · Ye, Mingliang
Journal of the American Chemical Society 2025
Effectively distinguishes arginine dimethylation isomers in complex cell samples
Identified sDMA at R112 of SNRPN, confirmed modified by PRMT5
Feasibility verified by orthogonal validation with neutral loss