Hairy Root Transformation System

For Al-tolerance genes

An Agrobacterium rhizogenes-mediated system that rapidly generates transgenic hairy roots in Stylosanthes for functional analysis of aluminum-tolerance genes.

Jian-Yu, Zhang · Kun, Zhao · Pan-Dao, Liu · Luo, Lijuan · Liu, Pandao

Plant Physiology and Biochemistry 2025

Advantages

Overexpression of SgHAD1 significantly enhanced Al-tolerance in transgenic hairy roots

SgHAD1-overexpressing hairy roots showed decreased G6P and F6P and increased glucose and fructose

SgHAD1 localized in cytoplasm with high hydrolytic activity toward G6P and F6P